Abdalla Ashraf Nadiem, Amr Helmy El Bolok And Sherif Farouk El-Gayar ,

ABSTRACT
Background: The World Health Organization (WHO) Reported Oral Cancer As Having One Of The Highest Mortality Ratios Amongst All Malignancies. Sinapic Acid Is One Of The Phenolic Compounds Which Considered A Group Of Key Plant Metabolites Which Can Induce Apoptosis In Laryngeal Carcinoma Cells. Aim Of This Study: The Aim Of The Study Is To Investigate The Influence Of Sinapic Acid Versus Nano Sinapic Acid On Induction Of Apoptosis In Human Laryngeal Carcinoma Cell Line Using PCR.

Materials & Methods: Treatment Of Cell Line (HEp-2) By Different Concentrations Of Sinapic Acid And Nano-sinapic Acid Was Done To Assess The Viability Of The Treated Cells To Determine The Percentages Of Viable Cells That Was Monitored Using MTT Assay And To Asses Ahe Apoptotic Effect Of These Drugs Using Real Time PCR.

Results: The Mean Viability Percentage Of Sinapic Acid And Nano-sinapic Acid Treated Cells (in Relation To Control Cells) Increased As Sinapic And Nano-sinapic Acid Concentration Decreased From 1000 MM To 4.88mM In Which The IC50 Of Sinapic Acid Is 565.42 MM.and 644.15 For Nano-sinapic Acid. There Is Increase In Fold Change In Apoptotic Genes (caspase And P53) Which Indicate The Apoptotic Activity Of Sinapic And Nano-sinapic Acid. It Was Concluded That Sinapic Acid Induce Apoptosis And Change Cancer Cell Morphology Due To Their Pro-apoptotic Activity.